High binding surface on High Binding 96 Well Breakable Strip Plates is a hydrophilic surface suitable for passive adsorption of proteins with different grades of hydrophilicity. This surface is ideal for immunoassays with a binding capacity of 400 to 500 ng IgG/cm2.
Luminescence Assay (LIA)
Fluorescence Assay (FIA)
Chemiluminescent Assay (CLIA)
High Binding Capacity
96 Well Plates
High binding capacitsurfaces are used in assays where the adsorbed molecule exceeds (up to 400 ng/cm2) the complementary molecules that have to be detected.
Furthermore, this surface is highly selective and shows high affinity towards the adsorption of molecules also when those are present in very small amounts (<50 ng/cm2) allowing to obtain the maximum sensitivity of the test.
High binding surfaces have different possible fields of application:
Protein – Antibody Anti-IgM adsorbed onto solid phase used in IgM capture assays
Lipo-protein – Rubella antigen adsorbed onto solid phase used in IgG assays
ELISA (competitive) tests for Steroid Hormones and TSH
A test simulating a competitive method shows the performance of this surface.
see technical note
Comparison of different types of High Binding capacity polystyrene strips.
see technical note
Biomat ‘s High Binding Capacity surfaces are available on the following Biomat’s products:
96 well plates:
High Binding 96 Well Solid Plates
High Binding 96 Well Strip Plates
High Binding 96 Wells Breakable Strip Plates
Moreover, it is offered on the client’s own products.
Microplates show a CV% less than 5 using:
HIgG as a coating molecule in an ELISA format, anti HIgG-HRP as detector and TMB as substrate
Biotinylated albumin as a coating molecule in an ELISA format, streptavidin-HRP as detector and TMB as substrate
The microplates are stored at room temperature in sealed PE bags.
High Binding Capacity Microplates
High Binding Capacity 96 Well Plates 8 Well Breakable Strip
High Binding Capacity 96 Well Plates 8 Well Strip
High Binding Capacity Solid 96 Well Plates