Additional information
Catalog Number | #6800S |
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Details | 100 rxns á 50 µl, contains: Volcano3G® RT‐PCR Probe 2x Master Mix (2x 1.25 ml) + Duplex Primer/Probe Mix (1x 1.3 ml) |
Downloads |
$480.00
Acceleration of SARS-CoV-2 testing: Simply skip the step of RNA extraction.
The kit contains Volcano3G® RT-PCR Probe 2x Master Mix and a duplex primer/probe mix for both SARS-CoV-2 (N1 gene specific) and RNaseP control.
To learn more about the use of SARS-CoV-2 RT-PCR detection kit w/o RNA extraction, please take a look at the procedure detailed in our recent reference: “Detection of SARS-CoV-2 from raw patient samples by coupled high temperature reverse transcription and amplification”
This publication shows:
“The approach delivers results without the need to purify RNA, reduces handling steps, minimizes costs, and allows evaluation by non-specialized equipment. The use of unprocessed swap samples is enabled by employing a heat-stable RNA- and DNA-dependent DNA polymerase, which performs the double task of stringent reverse transcription of RNA at elevated temperatures as well as PCR amplification of a SARS-CoV-2 specific target gene .” (Quote from the publication)
The University of Konstanz is currently using an adapted procedure employing the Direct SARS-CoV-2 RT-PCR detection kit w/o RNA extraction in a campus-wide surveillance study.
Please note: For research use only. For clinical testing, you may use this assay as a lab developed test validated by you following the applicable regulatory requirements.
Catalog Number | #6800S |
---|---|
Details | 100 rxns á 50 µl, contains: Volcano3G® RT‐PCR Probe 2x Master Mix (2x 1.25 ml) + Duplex Primer/Probe Mix (1x 1.3 ml) |
Downloads |