484-588-5452
[email protected]
My Account
Product has been added to your cart.

DirectBlood Genotyping PCR Kit. 100 reactions à 20 µl, 4x LyoCakes

DirectBlood Genotyping PCR Kit. 100 reactions à 20 µl, 4x LyoCakes

$235.00

DirectBlood Genotyping Kit (#5000) performance with a hydrolysis probe assay

The DirectBlood Genotyping Kit is compatible with a wide range of assays: Here we are detecting a SNP in the Factor V gene (rs6025; G1691A), which is connected with a higher risk for venous thrombosis, with a fluorescent hydrolysis probe assay.

 

 

SKU: myPOLS 5000S Categories: ,

Description

DirectBlood Genotyping Kit (#5000) performance with a hydrolysis probe assay

 

The DirectBlood Genotyping Kit is compatible with a wide range of assays: Here we are detecting a SNP in the Factor V gene (rs6025; G1691A), which is connected with a higher risk for venous thrombosis, with a fluorescent hydrolysis probe assay.

Homozygous wild type blood samples will show a strong signal and amplification plot (blue curve) in channel 2 (Cy5) and none or a very low signal

(red curve) in the other channel 1 (ROX).

 

 

Heterozygous blood samples will give an intermediate signal and amplification plots with very similar intensities in both channels 1 and 2 (blue and red curve).    Homozygous mutant blood samples, channel 1 (ROX) will show a strong signal and amplification plot (red curve) and channel 2 (Cy5) none or a very low signal and amplification plot (blue curve). So the signals are reversed on homozygous mutant samples.

Additional information

Content

DirectBlood Genotyping PCR Kit contains all components necessary for direct PCR or PCR based genotyping directly from blood samples including an engineered DNA polymerase, an specifically optimized reaction buffer system, and ultrapure dNTPs. An aptamer-based hot-start formulation of the included blood-resistant DNA polymerase prevents false amplification. Temperatures above 50°-55°C cause the aptamer’s secondary structure to melt and will set-free the polymerase. Only target specific primers and probes need to be added.
Publications

Link 1 to a relevant publication
https://www.sciencedirect.com/science/article/pii/S0009898118305886?viaihub

Link 2 to a relevant publication
https://link.springer.com/article/10.1007s11033-019-04696-9
Storage

The freeze-dried DirectBlood Genotyping PCR Lyocake can be stored at room-temperature. Please store resuspended master mixes (2x) and primer/probe mixes (4x) at -20°C. Minimize the number of freeze-thaw cycles by storing in aliquots. For a day-to-day use, we recommend keeping an aliquot at 4°C. Please store the included rehydration buffer and additional primer/probe mixes upon arrival at -20°C.
Manual

https://cdn.shopify.com/s/files/1/0226/4750/4971/files/5000_DirectBlood_Genotyping_PCR_Kit_web_v5.pdf?1035
Catalog Number

#5000S