Express DNA Ligation Kit (50 units)

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Express DNA Ligation Kit (50 units)

$57.90

The novel metagenome derived DNA Ligase offers flexible cloning of target genes into vector DNA molecules regardless of their sizes in base pairs. This enzymes is highly stable for long periods and still active even after three (3) years of storage. It is highly suitable for difficult to clone genes, any restriction endonuclease digested DNA, be it sticky or blunt-ends. The enzyme can function in any buffer background and often does not necessarily require buffers with ATP molecules. This means researchers can add the enzymes immediately after restriction endonuclease digestions post deactivation steps without cleaning up the mixtures. More importantly this enzyme ligate DNA molecules within five (5) to fifteen (15) minutes in optimal conditions. However to achieve complete ligated mixtures, 30 minutes incubation will be required.

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Description

Conventional DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5′-phosphate and 3′-hydroxyl termini in duplex DNA, RNA or DNA/RNA hybrids. All DNA ligase require ATP as a co-factor. However, the CapeBio Express DNA Ligase has been demonstrated to ligate DNA molecules without using ATP as a co-factor, enabling it to function in any buffer background. Moreover, it has shown to be a highly stable enzyme that exhibits activity even after three years of freeze-thawing.

The Express DNA Ligation Kit is designed for these applications:

  • Cloning of restriction enzyme- generated DNA fragments
  • QuickChange or Site-directed mutagenesis
  • Cloning of PCR generated products
  • Amplified fragment length polymorphism
  • Self-circularization of linear DNA
  • Joining of double-stranded oligonucleotide linkers or adapters to DNA
  • Nick repair in duplex DNA, RNA or DNA/RNA hybrids

Additional information

Catalog Number

Exp050

Kit Components

Express DNA Ligation Kit
10x Express Ligation buffer A
5x Express Ligation buffer B
Extra-pure PCR Grade Water

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