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GreenDye, 20x, 200 rxn á 25 µl, 1x 250 µl

GreenDye, 20x, 200 rxn á 25 µl, 1x 250 µl

$60.00

Boost your qPCR results without inhibition with GreenDye!

GreenDye is a highly sensitive DNA binding dye suitable for qPCR applications and melting curve analysis.
GreenDye shows very low DNA polymerase inhibition when compared with other dyes in PCR. It features a very high fluorescence when bound to double-stranded DNA and exhibits no degradation during PCR cycling. It is a perfect dye for qPCR and high resolution melting applications.

SKU: myPOLS 2000S Categories: ,

Additional information

Applications

– real-time PCR (FAM channel)
– melt curve analysis
– High Resolution Melting (HRM)
– qPCR
– real-time primer extension
– screening

It is recommended to use GreenDye at 1x concentration.
For example, add 1.25 μl GreenDye to a 25 µl PCR reaction. The fluorescence is measured at the usual SYBR/FAM channels.
Storage

This product is shipped in cool packs. Please store the product upon arrival at – 20 °C. Minimize the number of freeze-thaw cycles by storing in aliquots. For a day-to-day use, we recommend keeping an aliquot at 4 °C.
Downloads

Manual:
https://cdn.shopify.com/s/files/1/0226/4750/4971/files/2000_GreenDye_20X_fluorescent_nucleic_acid_dye__web_v3.pdf?961
Quality

GreenDye 20x is tested together with HiDi DNA polymerase (#9001) in a real-time PCR. A sequence in genomic HeLa DNA is used as a target to validate ct- and fluorescence values of GreenDye at 1x concentration.
Please inquire more information at [email protected] for lot-specific information.
No contamination has been detected in standard test reactions.
Catalog Number

#2000S