Using HiDi, less than 10 mutations can be detected in a background of >10.000 wild-type sequences without any other modifications and tricks on the assay.

HiDiTaq works also with hydrolysis probes (Taqman etc.)

HiDi is the ideal enzyme for mutation detection and quantifications.

Benchmarking clearly shows that HiDi is the gold standard for mutation detection.

HiDi and HiDi Taq DNA polymerase are also highly suitable for CRISPR-Cas or TALEN induced mutation identifications.

HiDi DNA polymerase is the gold-standard enzyme, whenever High Discrimination (hence the name “HiDi”) is required. Benchmarking data with other well-known enzymes on the market show that HiDi is the best choice for highly selective PCRs, such as allele-specific PCR, HLA genotyping, analysis of single CpG methylation sites or the detection of mutations in a high background of wild-type sequences.
Whereas many DNA polymerases tolerate mismatched primer-template complexes, HiDi DNA polymerase efficiently discriminates those and produces specific amplicons in case of perfectly matched primer pairs.

The HiDi Taq variant has a 5′-3′-nuclease activity and therefore is suitable for hydrolysis probe-based assays.