HiDi Taq DNA Polymerase, 250 U, 5 U/µl, 50 µl, including separate reaction buffer

HiDi Taq DNA Polymerase, 250 U, 5 U/µl, 50 µl, including separate reaction buffer


Using HiDi, less than 10 mutations can be detected in a background of >10.000 wild-type sequences without any other modifications and tricks on the assay.

HiDiTaq works also with hydrolysis probes (Taqman etc.)

HiDi is the ideal enzyme for mutation detection and quantifications.

Benchmarking clearly shows that HiDi is the gold standard for mutation detection.

HiDi and HiDi Taq DNA polymerase are also highly suitable for CRISPR-Cas or TALEN induced mutation identifications.

HiDi DNA polymerase is the gold-standard enzyme, whenever High Discrimination (hence the name “HiDi”) is required. Benchmarking data with other well-known enzymes on the market show that HiDi is the best choice for highly selective PCRs, such as allele-specific PCR, HLA genotyping, analysis of single CpG methylation sites or the detection of mutations in a high background of wild-type sequences.
Whereas many DNA polymerases tolerate mismatched primer-template complexes, HiDi DNA polymerase efficiently discriminates those and produces specific amplicons in case of perfectly matched primer pairs.

The HiDi Taq variant has a 5′-3′-nuclease activity and therefore is suitable for hydrolysis probe-based assays.

SKU: myPOLS 9201S Categories: ,

Additional information


HiDi and HiDi Taq DNA polymerase is hotstart-formulated with an aptamer. Temperatures above 50-55°C cause the aptamer’s secondary structure to melt and will set-free the polymerase.
HiDi is supplied as a 5 U/µl solution containing glycerol and is supplied together with 10x reaction buffer which has been optimized for short amplicons between 50-200 bp of length.
The buffer contains 2.5 mM Magnesium-ions in the final 1x concentration. Additional Magnesium (+ 0.5 – 1.5 mM) might be added in case of longer amplicons >500 bp.
HiDi DNA polymerase can also be used for real-time cycling, when adding a suitable real-time dye, for example GreenDye (#2000).


This product is shipped on cool packs. It is recommended to store the product upon arrival at -20°C.


HiDi Manual:

HiDi Taq Manual:

HiDi Taq benchmarking data:

HiDi application note:


HiDi DNA polymerase is tested for successful ASA performance detecting a genomic SNP (rs72921001) in HeLa genomic DNA. PCR products were subsequently analysed on a 2.5% agarose gel. A specific product was visualized by ethidium bromide staining at the correct amplicon length of 109 bp for the matched primer. In case of the mismatch primer no product formation was observed after 50 cycles.

HiDi Taq DNA polymerase was tested successfully for hydrolysis probe based real-time PCR. The product demonstrated linearity of amplification over a specified serial dilution of human genomic DNA.

The activity of HiDi and HiDi Taq DNA polymerase was monitored and adjusted to a specific DNA polymerase activity using an artificial DNA template and a DNA primer. Enzyme concentration was determined by protein-specific staining. Please inquire more information at [email protected] for the lot-specific concentration. No contamination was detected in standard test reactions.

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