HiDi and HiDi Taq DNA polymerase is hotstart-formulated with an aptamer. Temperatures above 50-55°C cause the aptamer’s secondary structure to melt and will set-free the polymerase.
This product is shipped on cool packs. It is recommended to store the product upon arrival at -20°C.
HiDi Taq benchmarking data:
HiDi application note:
HiDi DNA polymerase is tested for successful ASA performance detecting a genomic SNP (rs72921001) in HeLa genomic DNA. PCR products were subsequently analysed on a 2.5% agarose gel. A specific product was visualized by ethidium bromide staining at the correct amplicon length of 109 bp for the matched primer. In case of the mismatch primer no product formation was observed after 50 cycles.
HiDi Taq DNA polymerase was tested successfully for hydrolysis probe based real-time PCR. The product demonstrated linearity of amplification over a specified serial dilution of human genomic DNA.
The activity of HiDi and HiDi Taq DNA polymerase was monitored and adjusted to a specific DNA polymerase activity using an artificial DNA template and a DNA primer. Enzyme concentration was determined by protein-specific staining. Please inquire more information at [email protected] for the lot-specific concentration. No contamination was detected in standard test reactions.