The Biomat 96 Well Plates for Mouse Monoclonal Antibodies Isotyping product is a 96 well microplate coated with:
goat anti mouse heavy chain (IgG subclasses 1, 2a, 2b, 3 and IgA and IgM classes)
Isotyping involves determining the class (e.g. IgG vs IgA) and subclass (e.g. IgG2a vs IgG2b) of a monoclonal antibody: this is a critical step in antibody production as it is necessary for choosing an appropriate purification and modification method for the antibody.
The microplate can be used for performing an ELISA strip-well plate with individual wells precoated with goat anti-mouse heavy chain capture antibody (anti IgG1, IgG2a, IgG2b, IgG3, IgA, IgM) and goat anti-mouse light chain capture antibody (kappa and lambda). A mouse monoclonal antibody sample applied to the wells can be isotyped within an hour and half. Results can be evaluated qualitatively by visual inspection or quantitatively by measuring the absorbance at 450 nm (i.e. if using a goat anti-mouse HRP conjugate as detector).
Features of Biomat coated plates for mouse monoclonal antibody isotyping :
eight well strip format allows a convenient partial use of the plate; use one strip (column) for each sample (12 samples per plate)