This mix contains a Taq DNA polymerase variant in reaction buffer and ultrapure dNTPs. The DNA polymerase is hotstart-formulated with an aptamer. Temperatures above 50-55°C cause the aptamer’s secondary structure to melt and will set-free the DNA polymerase. It can also be used for real-time cycling, when adding a suitable realtime dye, for example GreenDye (#2000), or a fluorescent probe.
This product is shipped on cool packs. It is recommended to store the product upon arrival at -20°C.
Taq 2x PCR Master Mix is tested for successful PCR performance. A 92 bp fragment (beta-actin gene) was amplified from human genomic DNA and analysed by agarose gel electrophoresis. The activity of Taq DNA polymerase was monitored and adjusted to a specific DNA polymerase activity using an artificial DNA template and DNA primer. Enzyme concentration was determined by protein-specific staining. Please inquire more information at [email protected] for the lot-specific concentration. No contamination was detected in standard test reactions.