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Taq 2x PCR Master Mix. 500 reactions á 50 µl, 10 x 1.25 ml

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Taq 2x PCR Master Mix. 500 reactions á 50 µl, 10 x 1.25 ml

$248.00

Taq 2x PCR Master Mix – ready to use mix simplifies your PCR setup. Only primers and template need to be added as the mix contains all copmponents for a successful and reliable PCR. This ensures reproducible results, significantly reduces set-up times and the risk of pipetting errors. The robustness of PCR performance allows the application of this mix in a wide range of PCR amplifications.

Broad Amplification Range

Different-sized amplicons from 3 ng of a DNA plasmid were amplified. The use of Taq DNA polymerase resulted in clean and high yield of products, as analysed after PCR on a 0.8% agarose gel.

Faster Detection and Higher Sensitivity

A fragment (64 bp) of the human blood-coagulation factor IIa (F2) was amplified from 20 ng, 2 ng, 200 pg and 20 pg of a human genomic DNA extract. The same experiment was performed in parallel using the Taq DNA polymerase mix from another well-established and known supplier. PCR products were subsequently analysed on a 2.5% agarose gel

SKU: myPOLS 2001M Categories: ,

Additional information

Content

This mix contains a Taq DNA polymerase variant in reaction buffer and ultrapure dNTPs. The DNA polymerase is hotstart-formulated with an aptamer. Temperatures above 50-55°C cause the aptamer’s secondary structure to melt and will set-free the DNA polymerase. It can also be used for real-time cycling, when adding a suitable realtime dye, for example GreenDye (#2000), or a fluorescent probe.
Storage

This product is shipped on cool packs. It is recommended to store the product upon arrival at -20°C.
Manual

https://cdn.shopify.com/s/files/1/0226/4750/4971/files/2001_Taq_2x_PCR_Master_Mix_Manual_compact_web_v3_59a7d070-796c-472c-9804-4e7bfe62bb40.pdf?1156
Quality

Taq 2x PCR Master Mix is tested for successful PCR performance. A 92 bp fragment (beta-actin gene) was amplified from human genomic DNA and analysed by agarose gel electrophoresis. The activity of Taq DNA polymerase was monitored and adjusted to a specific DNA polymerase activity using an artificial DNA template and DNA primer. Enzyme concentration was determined by protein-specific staining. Please inquire more information at [email protected] for the lot-specific concentration. No contamination was detected in standard test reactions.
Catalog Number

#2001M