Volcano3G RT-PCR Probe 2x MasterMix + ROX reference dye (High ROX), 500 reactions á 25 µl, 5×1.25 ml
Volcano3G RT-PCR Probe 2x MasterMix + ROX reference dye (High ROX), 500 reactions á 25 µl, 5×1.25 ml
$510.00
Volcano3G® RT-PCR master mixes enable amplification of RNA or DNA target sequences with quick and easy PCR protocols, even including 0-step amplification.
Additionally, the light blue color improves the visibility of the mix during pipetting steps especially when white or transparent well-plates are used.
The mix is suitable for both qPCR of DNA and RNA templates, for GreenDye or probe based assays.
And all of this for a highly compatible price comparable to a standard qPCR mix.
Volcano3G® RT-PCR Probe 2x Master Mix is a ready-to-use master mix for reliable qPCR and RT-PCR in all standard PCR cyclers. The mix contains an enzyme blend of a robust Taq DNA polymerase and an engineered, thermostable DNA polymerase with reverse transcriptase activity. It is optimized for probe based detection assays of both RNA and DNA targets including multiplex PCRs. This variant of the master mix is supplemented with ROX reference dye. It is available in two different ROX concentrations (high and low).
A new study, just published on a preprint server medrxiv shows that SARS-CoV-2 can also be detected from dry swabs without extraction steps using Volcano3G® link to paper.
Volcano3G® RT-PCR Probe 2x Master Mix contains an hotstart-formulated enzyme blend of an engineered, thermostable DNA polymerase with reverse transcriptase activity and a robust Taq DNA polymerase. The blend is pre-mixed with ultrapure dNTPs and buffer components to create a ready-to-use 2x master mix for reliable PCR detection of both DNA and RNA targets with hydrolysis probes. In this variant, the mix is supplemented with ROX as reference dye in either a high or low concentration.
Storage
This product is shipped on cool packs. It is recommended to store the product at -20°C upon arrival.
