Save time with 0-step protocols
Skip the reverse transcription step. Isothermal reverse-transcription steps are not needed.
Volcano2G is a PCR-active reverse transcriptase and extremely thermostable with a half-life at 95°C of >40 min
Sensitive RNA detection
Volcano2G DNA polymerase can also be used for real-time PCR, when adding a real-time PCR dye. Volcano2G has a Taq DNA polymerase-like nuclease domain making it also suitable for hydrolysis probe based assays. As demonstrated here, NONO mRNA was detected using a 10-fold dilution series of total RNA by using a probe-based qPCR assay and the zero-step RT-PCR protocol.
Direct RT-PCR from cells
Saves time and money
VolcanoCell2G RT-PCR 2x Master mix has a streamlined ‘zero-step’ workflow that allows you to circumvent laborious purification and extraction steps, saving you both time and money.
VolcanoCell2G RT-PCR 2x achieves detection sensitivities down to single cells. In this example, GAPDH mRNA was detected from a single Lund human mesencephalic (LUHMES) cell following the VolcanoCell2G protocol.
A publication with more examples can be found in the Publications section.